The many spheres in which CO2 chambers show their strengths

CO free2 incubators, there would be no coronavirus vaccines today. They are also absolutely essential for cancer research. These multiple uses save lives and cure many different diseases. We would now like to introduce you to some of the interesting facets of CO2 incubators.

Sponsored by BINDER GmbH.

Regenerative medicine

CO2 incubators are used to conduct research in laboratories around the world. The Middle East Institute of Bioscienceswhich is one of the world’s leading centers for regenerative medicine, also uses an incubator to treat the body’s own cells as well as for plastic surgery applications.

The cells, which multiply in an incubator, are also used for tissue repair as well as for orthopedic and dermatological treatments. The Bioscience Institute only uses skin and fat tissue samples from adult (mature) cells. Using the body’s own cells, i.e. autologous cells, eliminates the risk of rejection while preventing the complication of graft-versus-host disease (an adverse reaction of the donor’s immune cells).

To be even more precise: CO2 incubators are mainly used to incubate stem cells from mesenchymal tissue (undifferentiated connective tissue).

Here’s how it works: First, cells are extracted from fatty tissue. This process is carried out by means of enzymatic deaggregation (separation) using different stages of filtration and centrifugation. The crucial step here is expansion, i.e. the extraction of as many stem cells as possible, which is why it is absolutely essential to create the best possible growth conditions.

Dr. Simona Alfano, a biologist at the Institute of Biosciences, explained:

“When incubating cells, it is of vital importance that the selected parameters remain exactly constant at all levels.”

And this is precisely where the CO2 Chambers from BINDER come into their own – with their reproducible growth conditions, constant climatic conditions, low risk of contamination and high level of safety.

Find out why ph value is a key factor in cell and tissue cultures.

Utmost safety is essential when preparing stem cells. (Credit: BINDER)

The coronavirus pandemic

CO2 The chambers have also played an important role during the coronavirus pandemic: firstly, in the development of coronavirus vaccines and, secondly, in testing drugs that can be used to treat COVID-19 on cells.

For this work, large pharmaceutical companies needed huge volumes of cells, which they were able to acquire using an incubator. The newly developed active ingredients were then tested using the cells.

The new vaccines used in the fight against the coronavirus have also been repeatedly tested on cells in the laboratory and evaluated. An incubator has proven to be essential equipment in a lab, especially during the coronavirus pandemic.

Learn about high-end equipment for virus research.

Space biology

The Institute of Medical Engineering at Lucerne University of Applied Sciences and Arts conducted research in the field of space biology. The research team, led by Dr. Fabian Ille, is assisted in its work by a CO2 bedroom.

Cells from a bovine hoof are incubated inside the cabinet at regular intervals until needed for a specific experiment. Recently, the cells were frozen and transported to the French city of Bordeaux by Dr. Simon Wüest and a team of researchers.

The reason for this trip was that the Lucerne research team had been selected by the European Space Agency (ESA) to participate in parabolic flights over the Atlantic. Shortly before the parabolic flights, which lasted a total of three hours, the cells were removed from the incubator and moved to specially prepared flight equipment and placed under controlled temperature conditions.

The Lucerne scientists wanted to use parabolic flights to study how cells respond and adapt to mechanical forces. These findings will help them in future attempts to grow cartilage to a stronger and better consistency, for example. In other words, it might be possible to take cells from a patient, replicate them with this innovative new method, and then reuse them in treating human patients.

“The weightless conditions help us make significant progress,” said Dr Ille, reflecting on the research project so far.

In laboratory tests that have already been carried out, Wüest and Ille were able to demonstrate in very general terms that this process could work in the future. In these tests, weightlessness conditions were simulated using a random position machine. here again, a CO2 BINDER chamber was used.

Novespace.  (Source: Lucerne University of Applied Sciences and Arts)
Novespace. (Source: Lucerne University of Applied Sciences and Arts)

Requirements for CO2 incubators

Safety is the top priority here. Sterilization at 180°C ensures, for example, that each series of tests begins with a clean and completely sterile incubator. Plus, the fanless design means germs aren’t agitated.

The result is optimal cell growth and absolutely no contamination from airborne germs. A deep-drawn inner chamber with no corners or edges also makes thorough cleaning of the incubator easy. It is therefore not surprising that large pharmaceutical companies specifically choose to trust CO2 incubators from BINDER.

BINDER CO2 incubators are the perfect combination of a range of solutions: hot air sterilization at 180°C, rapid control, interiors without accessories and absolutely zero consumables. For optimal cell growth – safe, reliable, smart, economical – look no further than BINDER.

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